tracking the evil plant
Source Eubulides
Date 03/07/10/02:21
DNA profiles link dope to its source

Forensic scientists in the US are applying DNA fingerprinting methods to
the cannabis plant. They say the technique, which is being used to create
a database of DNA profiles of different marijuana plants, will help them
to trace the source of any sample.

"It links everybody together: the user, the distributor, the grower," says
the database's creator, Heather Miller Coyle of the Connecticut State
Forensic Science Laboratory in Meriden. "That's the real intent of it, to
show it's not just one guy with a little bag of marijuana, but it's a
group of people."

A method for spotting the tiniest traces of marijuana, based on detecting
DNA unique to cannabis chloroplasts, has already been developed in the UK
(New Scientist print edition, 7 August 1999). But the profiling method,
based on the same principles as DNA fingerprinting of people, can
distinguish between closely related cannabis plants (Croatian Medical
Journal, vol 44, p 315).

In a case awaiting trial in Connecticut, prosecutors plan to use cannabis
DNA profiles to show that two apparently separate cannabis growing
operations were actually linked. The two operations, in different parts of
the state appeared separate until analysis of the plants revealed that
some had identical DNA fingerprints, showing that the growers were sharing
material. "From the investigative point of view that was phenomenal," says
Timothy Palmbach, director of scientific services at the laboratory.

Potent plants
The big difference between human and plant DNA fingerprinting is that in
people, each fingerprint is almost certainly unique to one person. So if a
crime scene sample matches a person's profile, there is little doubt that
it came from that individual.

In plants, by contrast, identical clones are easily created by taking
cuttings, a method growers often use to perpetuate potent strains of dope.
So showing two samples have matching DNA profiles does not by itself prove
they come from the same grower, let alone the same plant. But Palmbach
says that growers tend not to give away cuttings of their best plants, so
linking samples in this way is an important lead for investigators and
will still be useful in tracing samples.

"What growers have done to get more potent plants has played right into
our hands," says Palmbach. And if several matching profiles are found in
separate samples, the chances are high that they are somehow linked.

Terrorist ties
Coyle is establishing a database of DNA profiles from hundreds of
marijuana samples seized in Connecticut. "We want to track how many
varieties are out there, what the trends in distribution are, the
probability that a plant can be related to another," says Palmbach. The
database is being extended to include samples from all over the US and the
rest of the world. "We invite anyone to send us samples," says Coyle.

Exactly how law enforcement agencies will apply the method remains to be
seen. If a link can be established between a user and a grower or dealer,
casual users might find themselves in deeper trouble than they bargained
for. "If you're buying marijuana from somebody with terrorist ties, it
could be traced back to that person," warns Gary Shutler of the Washington
State Patrol's crime laboratory division.

On the other hand, he says, where medical uses of marijuana are legal, the
technology could help characterise strains with the desired medicinal
properties. Several US states have voted to legalise the medical use of
cannabis, though these efforts are being fought by federal authorities.

The technology will not help police investigating the production or sale
of highly processed or synthetic drugs such as cocaine and ecstasy. Nor
does the team think it would work with hashish, which is made from resin
exuded by cannabis plants, as not enough cellular material can be
recovered. If the cannabis profiling technique does prove to be an
effective tool in investigations and in the courtroom, dealers may switch
to selling hashish.

Sylvia Pagán Westphal

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